Effect of cervical intervertebral fusion implanted with
nacre/polylactic acid artificial bone*☆

Abstract

AIM：To evaluate the effect of the cervical intervertebral fusion implanted with nacre/polylactic acid artificial bone in dogs.

METHODS： These experiments were accomplished in the Center of Laboratory Animal and Laboratory of Spine and Orthopedic Surgery Department in Nanfang Hospital of Southern Medical University from September 2004 to June 2005. Fourteen adult dogs were divided into four groups by random digits table. Experimental group: Four dogs were implanted with nacre/polylactic acid artificial bone; Allograft group: Four dogs were implanted with allograft iliac bone soaked in ethanol of 0.75 volume fraction; Polylactic acid group: Four dogs were implanted with high polymer polylactic acid disinfected by ethylene oxide; Control group: Two dogs were implanted with nothing. All groups were implanted in the cervical 5/6 or 6/7 intervertebral space and fixed with hand surgery mini-plates. X-ray and histopathological examinations were applied after 4, 8, 12 and 16 weeks of operation.

RESULT:All 14 dogs were analyzed in the results.①The effect of cervical intervertebral fusion was evaluated by X-ray and histopathological examinations, and the fusion degree of experimental group was worse than that of allograft group but better than that of polylactic acid group after 16 weeks of operation.②The histological examination showed that there were little amount of soft tissue in anterior artificial bone, which were nonspecific fibrous connective tissue. The nacre microparticle could be seen in the normal bone tissue around the artificial bone and were phagocytosed by foreign body giant cells. There was much osteoid in the compound materials and some of polylactic acid blocks was biodegraded.③The ground section of the bone showed that the new bone grew into the hole of nacre/polylactic acid artificial bone. The nacre microparticle had no influence to bone tissue.

CONCLUSION: The nacre/polylactic acid artificial bone has a better fusion effect in vivo and can be slowly degraded.

摘要
目的：观察珍珠层／聚乳酸人工骨植入家犬颈椎椎间的融合效果。
方法：实验于2004－09/2005－06在南方医科大学附属南方医院实验动物中心及脊柱骨病科实验室完成。选择成年家犬14只，随机数字表法分为4组，实验组(n =4)：在颈椎5/6或者6/7椎间植入珍珠层/聚乳酸人工骨；异体骨组(n =4)：在相同部位植入体积分数为0.75的乙醇浸泡的新鲜同种异体髂骨；聚乳酸组(n =4)：植入经环氧乙烷消毒的高分子聚乳酸材料；空白对照组(n =2)：未植入任何材料，上述各组均用手外科微型钢板内固定。分别于术后4，8，12 ，16周行X射线片及组织病理检查。
结果：纳入动物14只，均进入结果分析。①通过X射线及组织病理检查对椎间融合进行判断，在术后16周，实验组的融合程度差于异体骨组，优于聚乳酸组。②病理检查显示复合材料前方的软组织量极少，为非特异性的纤维结缔组织，在周围正常骨组织内可见珍珠层粉微粒，细小的颗粒被异物巨细胞吞噬，复合材料中可见大量类骨质，材料中的聚乳酸部分降解。③骨磨片显示新骨组织长入复合材料的小孔内，珍珠层粉微粒对骨组织无明显影响。
结论：珍珠层／聚乳酸人工骨在体内有较好的植骨融合效果，并可以被缓慢降解吸收。
关键词：生物相容性材料；骨代用品；乳酸盐类

王建钧，陈建庭，杨春露，张登君，林焕阳.珍珠层/聚乳酸人工骨植入家犬颈椎椎间的融合效果[J].中国组织工程研究与临床康复，2007，11(1):41-44 [www.zglckf.com/zglckf/ejournal/upfiles/07-1/1k-41(ps).pdf]