两种仿生双相陶瓷生物骨的异位成骨作用
臧继传，修晓光，曹 斌，刘永辉
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Heterogeneous ossification of two kinds of bionic
biphasic ceramiclike biologic bones
Abstract
AIM：To discuss the new bone formation ability of biphasic ceramic-like biologic bone (BCBB) compounded with bone morphogenic protein (BMP) and typeⅡ collagen, which are transplanted into the heterogenous muscle pouches in rabbits.
METHODS： The experiment was carried out in the Institute of Orthopaedics, the Fourth Military Medical University of Chinese PLA between October 2004 and October 2005. A total of 32 healthy Japanese rabbits were randomly allocated into biologic active bone group and biologic bone group, each containing 16 rabbits. BCBB compounded with BMP and type Ⅱ collagen was called bionic biphasic ceramic-like biologic active bone and bionic BCBB, respectively. Two materials were transplanted into rabbit muscle pouches of two groups, then were harvested at weeks 2, 4, 8, 12 for the gross observation under microscope. Tissue sections were selected for hematoxylin-eosin (HE) staining and Masson staining.
RESULT:Totally 32 rabbits were involved in the result analysis.①Gross observation: At week 2, lamellar connective tissues amicula appeared on the surface of materials and formed new vascular net; At weeks 4, 6, 8, there were more vascular net and white bony tissues in biologic active bone group than in biologic bone group.②Staining results: At week 2 post-operation, HE staining demonstrated a great quantity of connective tissues on the surface and pore of materials, with the abundant blood capillary, but no new bone formed; At week 8, Masson staining demonstrated the scattered osteoid, which were blue new bone; At week 12, HE staining result showed that membrane bone formed on edge of materials, osteoblast arranged in lines while new bone collagen fiber in disorder, with the obvious osseous cells and bone lacuna. Whereas in biologic bone group, bionic BCBB had no ossification ability at week 12 post-operation, accompanying blue collagen fiber in mesh.
CONCLUSION: Both of two materials have good biocompatibility. Bionic biphasic ceramic-like biologic active bone has heterogenous ossification ability and bionic BCBB is ineffective in bone induction.
Department of Orthopaedics, Hiser Medical Center of Qingdao, Qingdao 266003, Shandong Province, China
Zang Ji-chuan, Attending physician, Department of Orthopaedics, Hiser Medical Center of Qingdao, Qingdao 266003, Shandong Province, China
xxg604@126.com
Received: 2006-06-15
Accepted: 2006-10-23
摘要
目的：将复合胶原、骨形态发生蛋白后的双相陶瓷生物骨支架材料异位肌袋内植入，观察新骨形成能力。
方法: 实验于2004－10/2005－10在第四军医大学全军骨科研究所及青岛医学院中心实验室完成。选择成年健康日本大耳兔32只，随机数字表法分为生物活性骨组和生物骨组，每组16只。将双相陶瓷生物骨支架材料分别与胶原、骨形态发生蛋白复合,制得仿生双相陶瓷生物骨和仿生双相陶瓷生物活性骨, 将两种材料分别植入生物活性骨组和生物骨组兔股部肌袋内, 分别于术后第2，4，8，12周取材, 进行植入材料的大体观察，组织切片分别做苏木精－伊红染色、Masson染色。
结果：纳入兔32只，均进入结果分析。①大体观察：生物活性骨组和生物骨组术后2周时，材料表面均形成薄层结缔组织包膜，包膜内形成新生的血管网；术后4，6，8周时生物活性骨组包膜内血管网及白色骨样组织生成多于生物骨组。②两组兔骨组织染色观察：生物活性骨组术后2周苏木精－伊红染色可见材料表面及孔隙有大量纤维结缔组织，毛细血管丰富，未见骨形成；术后8周Masson染色可见散在分布的类骨质，散在的蓝色组织为新生骨；术后12周苏木精－伊红染色可见材料边缘可见膜内成骨结构，可见排列成行的成骨细胞,新生骨胶原纤维排列稍紊乱,骨细胞及骨陷窝明显。生物骨组术后12周Masson染色材料边缘可见有蓝色网状分布的胶原纤维，无骨形成。
结论：两种材料肌袋植入后生物相容性好,仿生双相陶瓷生物活性骨具有异位成骨能力，仿生双相陶瓷生物骨无骨诱导作用。
关键词：仿生双相陶瓷生物骨；支架；组织工程
臧继传，修晓光，曹斌，刘永辉.两种仿生双相陶瓷生物骨的异位成骨作用[J].中国组织工程研究与临床康复，2007，11(1):45-47
[www.zglckf.com/zglckf/ejournal/upfiles/07-1/1k-45(ps).pdf]
青岛市海慈医疗集团骨外科，山东省青岛市 266003
臧继传，男，1954年生，山东省青岛市人，汉族，1990年青岛医学院毕业，主治医师，主要从事骨组织工程的研究。
xxg604@126.com
中图分类号:R318.08 文献标识码:A
文章编号:1673-8225
(2007)01-00045-03
收稿日期：2006-06-15
修回日期：2006-10-23
（06-50-6-4867/Y·Y）
课题背景：该课题系云南省教育厅科学研究基金资助项目（02QY126），课题选用猪椎骨为原材料进行理化处理，制备出类似人体骨组织天然结构和性能的材料。
创新要点：参考仿生的原理将双相陶瓷生物骨与Ⅰ型胶原、骨形态发生蛋白复合，制得仿生双相陶瓷生物骨和仿生双相陶瓷生物活性骨，使材料具有和天然骨基质类似的结构和功能。然后将两种材料异位肌袋移植，排除原位骨床干扰，确定两种材料成骨作用的有效性和异位构建骨组织的可能性。本实验中肌袋包埋8周时，仿生双相陶瓷生物活性骨可见异位新骨形成，有较明显的诱导异位成骨能力。而仿生双相陶瓷生物骨在观察时间内未见异位骨形成，说明该材料无骨诱导性。
同行评价：组织工程材料的生物活性和骨诱导性是目前的研究热点和重点，该文将猪椎骨经复合焦磷酸钠两次低温煅烧后制得组织工程支架双相陶瓷材料，并将胶原、骨形态发生蛋白与该材料复合制成复合生物骨后研究其异位成骨作用。实验证明这种新的生物衍生复合材料具有异位成骨作用。文章简洁，条理清
晰，对该类材料的实验研究有很好的参考价值。
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