Research and development of the Digital Stroke
Unit System in net version

Abstract

To observe the biocompatibility of the poly(lactide-co-glycolide) (PLGA) scaffold with the spinal cord tissue of rats. The experiment was performed at the Laboratory of Trauma Repair, Beijing Institute of Neurosurgery, Capital Medical University between May and September 2005. Totally 8 healthy adult female Wistar rats with the body mass of 250-300 g were selected. The PLGA was implanted into the injured site of T9 hemisected spinal cord injury (SCI) models. 3 mm spinal cord was removed, and PLGA scaffold was implanted in the spacing. Twice artificial urination was carried out every day after operation with paraplegina nursing care. The degradation of scaffold and the growth of tissue cells were observed under electron microscope at different time point at weeks 2, 4, 8 and 12 after transplantation, respectively. A total of 8 rats were involved in the result analysis. Scanning electron microscope showed that the materials degraded gradually with the prolongation of time after transplantation. Transmission electron microscope showed that at week 2 there were fibrocytes, fibroblasts and macrophage necrotic Schwann’s cells in the surface of materials. At weeks 4 and 8 regenerative thin-myelinated fibers and disintegrative or degenerative myelin sheath appeared; the number of macrophage necrotic Schwann’s cell was fewer than that at week 2. At week 12 regenerated medullated nerve fibers, fibrocyte and fibroblast adhered to the surface of materials; few macrophage necrotic Schwann’s cells appeared. The PLGA is a good scaffold material for spinals cord of rats with good biocompatibility.

摘要
观察生物可降解材料乙交酯－丙交酯共聚物支架与大鼠脊髓组织的生物相容性。实验于2005－05/09在首都医科大学附属北京市神经外科研究所损伤修复实验室完成。取健康成年雌性Wistar大鼠8只，体质量250～300 g，将组织工程材料乙交酯－丙交酯共聚物移植入大鼠T9水平脊髓半横断损伤处，切除脊髓3 mm，乙交酯－丙交酯共聚物支架移植入空隙处，术后每日人工排尿2次，截瘫护理。分别在移植后2，4，8，12周不同时间点电镜下观察支架的降解及组织细胞的生长状况。纳入8只大鼠，均进入结果分析。扫描电镜观察髓内移植后随时间的延长，材料逐步降解，透射电镜观察2周时可见材料表面有纤维细胞、纤维母细胞和巨噬细胞吞噬坏死变性的许旺细胞。 4周和8周时可见再生的薄髓纤维，崩解或变性髓鞘，巨噬细胞吞噬坏死变性的许旺细胞较2周时少。12周时材料表面附着再生的有髓神经纤维、纤维细胞和纤维母细胞，巨噬细胞吞噬坏死变性的许旺细胞少见。乙交酯－丙交酯共聚物支架在大鼠脊髓内具有良好的生物相容性。
关键词：组织工程；生物相容性材料；脊髓

陈刚，万虹，扬飞，历俊华，李德志，屈雪，孙异临，王身国，王忠诚.乙交酯－丙交酯共聚物与大鼠脊髓组织的生物相容性[J].中国组织工程研究与临床康复，2007，11(1):176-177 [www.zglckf.com/zglckf/ejournal/upfiles/07-1/1k-176(ps).pdf]