Influence of transplanting microencapsulated ovarian cells into the abdomen on the bioactivity of pancreatic islet alpha and beta cells in mice *★

Abstract

AIM：To observe the effect of transplanted microencapsulated ovarian cells on the biological function of pancreatic islet cells.

METHODS： The experiment was conducted in the Center of Reproductive Medicine, Capital Medical University from July 2005 to September 2006. Thirty adult female ICR mice were randomly divided into control group, ovariectomy group and transplantation group with 10 mice in each group. The bilateral ovaries of the ovariectomy group were extirpated via back incision; in the transplantation group, the microencapsulated ovarian cells were transplanted into the abdomen after bilateral ovariectomy. The control group mice were normally feed for 10 days without any operation. The mice of the ovariectomy group and transplantation group were killed at the 60th day after operation to take their blood samples and pancreatic tissue. Serum estrogen (E2) quality concentrations of three groups were tested by radioimmunoassay. Pancreas tissues were embedded by paraffin and sectioned to detect the glucagon, insulin, insulin receptor α and insulin receptor substrate -2 by immunohistochemistry. The average absorbance value of the positive areas was measured with image analyzer.

RESULT: All 30 mice were involved in the result analysis. ①Serum estrogen quality concentration of the ovariectomy group was lower than the control group and transplantation group significantly [(1.089±0.885), (37.582±20.019), (35.766±13.313) ng/L, t =5.759, 8.219, P < 0.01], but there was no significant difference in the serum estrogen level between the transplantation group and the control group. ②The glucagons and the positive products (A) of the ovariectomy group were significantly higher than those of the control group (1.282±0.076, 1.038±0.053; 1.023±0.055, 0.822±0.090, P < 0.01); there was no significant difference between the transplantation group and the control group; the insulin receptor α and positive products (A) of insulin receptor subtrate-2 in the ovariectomy group were significantly lower than those of the control group (0.929±0.142, 1.099±0.098; 0.891±0.118, 1.135±0.069, P < 0.01), but there was no significant deference between the transplantation group and the control group.

CONCLUSION:Ovariectomy combined the transplantation of microencapsulated ovarian cells could improve the quality concentration of serum estrogen, and effectively regulate the bioactivity of pancreatic islet α and β cells.

Zhou JL, Xu Q, Jin J, Shi XL.Influence of transplanting microencapsulated ovarian cells into the abdomen on the bioactivity of pancreatic islet alpha and beta cells in mice.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2007;11(13):2425-2429(China)
[www.zglckf.com/zglckf/ejournal/upfiles/07-13/13k-2425(ps).pdf]

摘要
目的：观察微囊化卵巢细胞种植于去卵巢小鼠腹腔后对胰岛细胞生物活性的影响。
方法：实验于2005－06/2006－09在首都医科大学生殖医学研究中心完成。将30只成年雌性ICR小鼠随机分为3组，正常对照组、卵巢摘除组和微囊种植组，每组10只。卵巢摘除组经背部切口取出双侧卵巢；微囊种植组于卵巢摘除同时，将微囊化卵巢细胞种植到腹腔内。正常对照组于饲养第10天，卵巢摘除组和微囊种植组于术后60 d处死采血并取出胰腺组织。以放射免疫法测定各组实验动物血清中雌二醇的质量浓度。胰腺组织分别应用免疫组织化学法标记胰高血糖素、胰岛素、胰岛素受体α和胰岛素受体底物2。实验结果用图像分析仪进行吸光度值的测定。
结果：30只小鼠均进入结果分析。①卵巢摘除组血清雌二醇的质量浓度低于正常对照组、微囊移植组，差异有非常显著性意义[分别为（1.089±0.885），（37.582±20.019），（35.766±13.313）ng/L，t =5.759，8.219，P < 0.01]，正常对照组与微囊种植组之间差异无显著性意义。②卵巢摘除组胰高血糖素、胰岛素的阳性产物（A）显著高于正常对照组，差异有显著性意义(分别为1.282±0.076，1.038±0.053; 1.023±0.055, 0.822±0.090, P < 0.01)，微囊种植组与正常对照组比较差异无显著性意义；卵巢摘除组胰岛素受体α和胰岛素受体底物2的阳性产物（A）显著低于正常对照组，差异有显著性意义?穴分别为0.929±0.142，1.099±0.098; 0.891±0.118，1.135±0.069, P < 0.01)，微囊种植组与正常对照组比较差异无显著性意义。
结论：在去除卵巢的同时进行了微囊化卵巢细胞的种植，可明显提高血清雌二醇的质量浓度，较有效地调节胰岛α细胞和β细胞的生物活性。
关键词：卵巢；胰岛；种植；微囊；激素替代疗法

周金玲，许晴，金洁，史小林.微囊化卵巢细胞植入卵巢摘除小鼠腹腔对胰岛α和β细胞生物活性的影响[J].中国组织工程研究与临床康复，2007，11(13):2425-2429 [www.zglckf.com/zglckf/ejournal/upfiles/07-13/13k-2425(ps).pdf]