Evaluation of adriamycin-induced cardiomyopathy in rabbits: Quantitative tissue velocity imaging versus transthoracic echocardiography

Abstract

AIM：To evaluate quantitative tissue velocity imaging (QTVI) parameters in detecting adriamycin (ADR)-induced cardiomyopathy model in rabbits, and compare with the conventional transthoracic echocardiography.

METHODS：The experiment was conducted in Dalian Medical University from June 2005 to August 2006.①Twenty-two New Zealand purebred white rabbits were used without limitation between female and male. Those rabbits were stochastically divided into two groups. Twelve rabbits in ADR group were injected with 2 mg/kg ADR intravenously, 1 g/L once a week for 8 weeks. Ten rabbits in control group received 2 mL/kg normal saline once a week for 8 weeks.②Conventional echographic parameters (the left ventricular inner diameter at end-systolic and end-diastolic phases, interventricular septal thickness, and left ventricular E peak velocity, ejection fraction, shortening fraction as well) were measured every week with HP Sonos 5500 Color Doppler Ultrasonograph (Agilent Company). QTVI parameters including peak velocity (Vp) at systolic and diastolic phases, velocity acceleration (Va) of systolic phase, were obtained every week with GE Vivid 7 Color Doppler Ultrasonograph (GE Company).

RESULT: All of the 22 rabbits were involved in the result analysis.①There was no significant difference in the parameters of control group and ADR group from the 1st to 12th weeks (P < 0.05).②At the 4th week, Vp and Va were significantly lower than those before injection in ADR group (P < 0.05).③At the 7th week, Vp and Va were significantly lower than those before injection in ADR group (P < 0.01), and E velocity decreased significantly (P < 0.05).④At the 8th week, Vp, Va and E velocity were all significantly lower than those before injection in ADR group (P < 0.01). The left ventricular inner diameters at end-systolic and end-diastolic phases in ADR group were enlarged significantly (P < 0.05).⑤At the 12th week, Vp and Va were significantly lower than those before injection in ADR group (P < 0.01), and left ventricular inner diameters at end-systolic and end-diastolic phases enlarged significantly (P < 0.01), interventricular septal thickness and left ventricular posterior wall thickness weakened significantly (P < 0.01). Ejection fraction, shortening fraction and E velocity were significantly lower (P < 0.01).

CONCLUSION:QTVI is valuable to assess ADR-induced cardiomyopathy model in rabbits, and its sensitivity is superior to conventional echocardiography.

Li GS, Zhang Z, Ren WD, Cui HY, Xia DZ, Ma CY, Zou LJ.Evaluation of adriamycin?鄄induced cardiomyopathy in rabbits: Quantitative tissue velocity imaging versus transthoracic echocardiography.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2007;11(22):4279-4283(China) [www.zglckf.com/zglckf/ejournal/upfiles/07-22/22k-4279(ps).pdf]

摘要
目的：采用定量组织速度成像技术评价阿霉素诱导兔心肌病模型，并与常规经胸超声心动图比较其评估优势。
方法：实验于2005－06/2006－08在大连医科大学完成。①实验分组及处理：取纯种新西兰白兔22只，雌雄不限，随机分成阿霉素组12只，给予阿霉素每次2 mg/kg，以1 g/L耳缘静脉注射，每周1次，注射8周；对照组10只每周注射2 mL/kg生理盐水，共8周。②实验评估：每周应用HP Sonos 5500型彩色多普勒超声诊断仪（美国 Agilent 公司生产）对两组兔心脏进行左室收缩末期和舒张末期内径明、室间隔厚度、E峰、射血分数、左室短轴缩短率等常规超声参数测量，使用GE Vivid 7型彩色多普勒超声诊断仪（美国 GE 公司生产）进行收缩期和舒张期峰值速度、收缩期加速度定量组织速度成像参数测定。
结果：22只兔进入统计。①对照组1～12周各参数与阿霉素组基础状态下比较无明显差异(P > 0.05)。②第4周阿霉素组二尖瓣环运动的平均收缩期和舒张期峰值速度、收缩期加速度较基础状态明显减低（P 均< 0.05）。③第7周阿霉素组二尖瓣环运动的收缩期和舒张期峰值速度、收缩期加速度较基础状态明显减低（P < 0.01），E峰较基础状态明显减低(P < 0.05)。④第8周阿霉素组二尖瓣环运动的收缩期和舒张期峰值速度、收缩期加速度较基础状态明显减低（P < 0.01），E峰较基础状态明显减低（P < 0.01），左室收缩末期和舒张末期内径明显增大（P < 0.05）。⑤第12周阿霉素组二尖瓣环运动的收缩期和舒张期峰值速度、收缩期加速度较基础状态明显减低（P < 0.01），左室收缩末期和舒张末期内径明显增大(P < 0.01)，室间隔厚度、左室后壁厚度明显变薄(P < 0.05)，射血分数、左室短轴缩短率和E峰明显减低（P < 0.01）。
结论：定量组织速度成像参数可有效评价阿霉素诱导心肌病模型兔心肌的病理变化，较常规超声参数更敏感。
关键词：定量组织速度成像；阿霉素；兔；心肌病；超声技术

礼广森，张卓，任卫东，崔洪岩，夏稻子，马春燕，邹丽娟.定量组织速度成像技术评价阿霉素诱导兔心肌病模型：与常规经胸超声心动图比较[J].中国组织工程研究与临床康复，2007，11(22):4279-4283 [www.zglckf.com/zglckf/ejournal/upfiles/07-22/22k-4279(ps).pdf]