课题背景：课题受两项国家自然科学基金资助，分别为“可降解生物材料与内皮细胞界面反应对细胞功能蛋白表达的影响及机理研究”（30370411）和“分子自组RAD16-RGD/CPP纳米仿生基质骨修复材料的研究”（50472091）。课题从新型骨修复材料聚磷酸钙出发，将锶元素掺入其中，与破骨细胞复合培养，观察掺锶聚磷酸钙对破骨细胞的形貌及生理功能的影响。

应用要点：①课题研究内容聚磷酸钙属于国际先进的新型生物材料。②课题组将锶元素掺入聚磷酸钙进行相关研究，国内属首次。③本实验为治疗骨组织缺损、缺失材料的研制提供新的思路及参考。

同行评价：文章观察了掺锶聚磷酸钙对破骨细胞的影响，属于生物材料领域一个比较新颖的研究课题。作者采用了同聚磷酸钙作对比的方法，从细胞的形态和生理功能两方面进行了考察，结果表明锶元素的掺入能通过降低破骨细胞的活性，阻碍其增殖分化，从而显著地抑制其骨吸收能力。讨论参考了大量的国外文献，有一定的针对性，具有较高的科研和应用价值。

摘要
目的：已有研究表明新型生物材料聚磷酸钙掺锶后能明显促进成骨细胞的增殖和分化。实验将掺锶聚磷酸钙与破骨细胞于体外复合培养，进一步从细胞的形态和生理功能两方面考察掺锶聚磷酸钙对破骨细胞的影响。
方法：实验于2006-10/2007-01在四川大学组织工程支架材料研究室及四川大学华西医学院生物医学工程重点实验室完成。将机械分离法获得的新生兔长骨破骨细胞分别种植于1 cm×1 cm×80 μm的骨片及由“湿式法”溶液反应法制备的φ2 mm×10 mm的掺锶聚磷酸钙样品上，以聚磷酸钙为对照，复合培养于24孔培养板中。复合培养7 d后，采用特异性抗酒石酸酸性磷酸酶染色方法鉴定实验细胞，通过扫描电镜观察骨片和掺锶聚磷酸钙、聚磷酸钙材料表面破骨细胞的形貌及骨吸收陷窝的形成情况。实验过程中对动物处置符合动物伦理学要求。
结果：①抗酒石酸酸性磷酸酶染色显示试验细胞呈阳性。②骨片上形成的骨吸收陷窝多呈不规则形状，陷窝边界清晰，底面粗糙。证实所培养的细胞为破骨细胞。③复合培养7 d后，掺锶聚磷酸钙组材料表面陷窝形成的数量明显少于聚磷酸钙组材料。④复合培养7 d后，掺锶聚磷酸钙组材料表面破骨细胞的增殖度明显低于聚磷酸钙组材料。
结论：掺锶聚磷酸钙由于锶元素的掺入能通过降低破骨细胞的活性，阻碍破骨细胞的增殖分化，从而显著地抑制其骨吸收能力。
关键词：掺锶聚磷酸钙； 破骨细胞； 骨吸收；生物材料；组织工程

冯婷，顾志鹏，任大伟，张小华，万昌秀，余喜讯．掺锶聚磷酸钙对破骨细胞的影响[J].中国组织工程研究与临床康复，2008，12(1):19-22 [www.zglckf.com/zglckf/ejournal/upfiles/08-1/1k-19(ps).pdf]

Effects of strontium-doped calcium polyphosphate on osteoclast

Abstract

AIM：Calcium polyphosphate as a new biomaterial can enhance the replication and differentiation of osteoblasts after being incorporated strontium. In this report, we will conduct a complex culture of strontium-doped calcium polyphosphate (SCPP) with osteoclasts in vitro, and further observe the effect of SCPP on figuration and physiological function of osteoclasts.
METHODS: The experiment was performed at the Tissue Engineering Scaffold Materials Laboratory and Huaxi Biomedical Engineering Laboratory of Sichuan University from October 2006 to January 2007. Osteoclasts obtained from new-laid rabbits by mechanical separation were compounded with 1 cm×1 cm×80 μm bone slices and φ2 mm×10 mm SCPP sample, which was prepared with solution reaction method, in 24-hole culture plate. CPP was used as the control. SCPP and CPP were produced by 'wet process'. Seven days after culture, tartrate-resistant acid phosphatase staining was applied to identify the cells. Osteoclast figuration and resorptive pits on bone slice, SCPP and CPP were viewed by scanning electron microscopy.
RESULTS: ①Cells presented masculine after tartrate-resistant acid phosphatase staining.②Resorptive pits with clear edge and rough undersurface appeared irregular shape on bone slices. Osteoclasts were identified.③Seven days after co-culture, the number of the resorptive pits on surface of SCPP was much smaller than that of CPP. ④Seven days after co-culture, the proliferation of osteoclasts on surface of SCPP was obviously lower than that of CPP.
CONCLUSION: SCPP can remarkably inhibit the osteoclastic bone resorption by reducing its activity, proliferation and differentiation because of the addition of strontium.

Feng T, Gu ZP, Ren DW, Zhang XH, Wan CX, Yu XX.Effects of strontium-doped calcium polyphosphate on osteoclast.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(1):19-22(China)
[www.zglckf.com/zglckf/ejournal/upfiles/08-1/1k-19(ps).pdf]