摘要
目的：观察羟基磷灰石在氯磷酸二钠表面改性前后润湿性变化。
方法：实验于2006-07/09 在口腔疾病研究国家重点实验室（四川大学）完成。①将羟基磷灰石制成10 mm×10 mm×2 mm大小的块状，共48 块，平均分为实验组与对照组。实验组与氯磷酸二钠复合制成氯磷酸二钠-羟基磷灰石；对照组用乙醇和蒸馏水超声波清洁，干燥备用。②配制质量浓度为0，20，40，60，80，100 g/L的小牛血清溶液各10 mL作为润湿剂。③以接触角测定仪测定各质量浓度小牛血清溶液在羟基磷灰石与氯磷酸二钠-羟基磷灰石表面的接触角，评估两组润湿性差异。
结果：不同质量浓度的小牛血清溶液在羟基磷灰石-氯磷酸二钠表面的接触角均小于单纯羟基磷灰石，差异有显著性意义（P < 0.01）。
结论：用二磷酸盐对羟基磷灰石进行表面改性可提高羟基磷灰石的表面润湿性。
关键词：二磷酸盐；羟基磷灰石；表面润湿性；生物材料

罗恩，胡静，李继华，梁新华，王雪梅，魏世成.羟基磷灰石经氯磷酸二钠表面改性后表面润湿性的变化[J].中国组织工程研究与临床康复，2008，12(10):1856-1858 [www.zglckf.com/zglckf/ejournal/upfiles/08-10/10k-1856(ps).pdf]

Effect of clodronate on wetting property of hydroxyapatite

Abstract

AIM：To investigate the changes of wetting property of hydroxyapatite with reshaped surface by clodronate.
METHODS: The experiment was completed in the State Key Laboratory of Oral Disease (Sichuan University) from July to September in 2007.①The hydroxyapatite was made into 48 pieces (10 mm×10 mm×2 mm) and then randomly divided into experimental group and control group with 24 pieces in each group. Sample in the experimental group was combined with the clodronate to form compound material, while sample in the control group was only cleaned with alcohol and distilled water under ultrasonic wave, and then dried for using.②Bovine calf serum solutions at different concentrations of 0%, 2%, 4%, 6%, 8%, 10% were prepared as wetting agents, each for 10 mL.③Contact angles of different concentrations of bovine calf serum solutions on hydroxyapatite and clodronate-hydroxyapatite compound were tested with the contact angle analyzer. And the wetting property of two groups was evaluated.
RESULTS: There were significant differences between the two groups with different concentrations of bovine calf serum solutions (P < 0.01), and the contact angle of hydroxyapatite group was higher than that of clodronate-hydroxyapatite group.
CONCLUSION: Clodronate modification of hydroxyapatite will increase wetting property of hydroxyapatite.

Luo E, Hu J, Li JH, Liang XH, Wang XM, Wei SC.Effect of clodronate on wetting property of hydroxyapatite.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(10):1856-1858(China)
[www.zglckf.com/zglckf/ejournal/upfiles/08-10/10k-1856(ps).pdf]