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[www.zglckf.com/zglckf/ejournal/upfiles/08-11/11k-2069(ps).pdf]

Influence of recombinant human platelet-derived growth factor plus insulin on burn wound healing in aging diabetic rats

Abstract

AIM£ºReports have shown that local and simple application of recombinant human platelet-derived growth factor (rhPDGF) or insulin could promote wound healing in diabetes. This study evaluated the effect of the combination of rhPDGF and insulin on burn wound healing in type 2 diabetic aging rats.
METHODS: The experiment was performed at the SPF Animal Experimental Laboratory of Gansu College of Traditional Chinese Medicine from October 2006 to May 2007. ¢ÙEighty-eight aging SPF Wistar rats of more than one year old, either female or male and 300-350 g were selected and randomly divided into burn control group (n =20), diabetic burn group (n =20), diabetic rhPDGF control group (n =16), diabetic insulin control group (n =16) and diabetic rhPDGF and insulin therapy group (n =16). Protamine Zina insulin was the product of Shanghai No. 1 Biochem. & Pharma. Co., Ltd. and rhPDGF was provided by Beijing Golden Sets Biopharmaceutical Technology Co., Ltd. ¢ÚExcept for the burn control group, all aging rats were fed with high-fat, high-protein and high-sugar food, then intraperitoneally injected with streptozotocin (STZ) to establish animal model of type 2 diabetes. Then the rats were still feed with high-fat, high-protein, and high-sugar food. After eight weeks, the rats in each group were given man-made deep second-degree burn. ¢ÛThe wound area was measured in 1, 3, 7, 14, and 21 days after burn to calculate the rate of wound healing. Morphology and hydroxyproline (OHP) content were observed. The expression of vascular endothelial growth factor (VEGF) and CD34 were detected by immunohistochemical staining to calculate microvascular density (MVD). The experimental procedure was accorded with animal ethical standards.
RESULTS: Compared with diabetic burn group, diabetic rhPDGF control group, and diabetic insulin control group, the wound healing of aging rats in diabetic rhPDGF and insulin therapy groups was significantly faster (P < 0.05), the epithelization was accelerated; moreover, OHP content, the expression of VEGF, CD34, MVD in wound tissue were significantly increased (P < 0.05).
CONCLUSION: The combination of rhPDGF and insulin in local wound tissue can accelerate the wound healing in aging diabetic rats by promoting wound collagen synthesis and increasing wound vasifaction.

Wu J, Xue XD, Liu JL, Si XQ, Yang GH, Liu JY.Influence of recombinant human platelet-derived growth factor plus insulin on burn wound healing in aging diabetic rats.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(11):2069-2073(China) [www.zglckf.com/zglckf/ejournal/upfiles/08-11/11k-2069(ps).pdf]