课题背景：选择生物相容性良好的聚合物覆盖在支架表面，可以克服金属支架本身的致血栓性，加速血管的内皮化过程。通过改进涂层支架的方法，还可以在支架表面直接涂层生物活性物质，发挥其抗血栓、抗炎等作用。本实验就是通过静电自组装的作用，在支架材料316L不锈钢表面制备丝素肽和壳聚糖聚合物涂层，对不锈钢进行表面改性。

重要的概念：层层静电自组装利用超分子静电自组装原理，通过静电力的作用依次吸附上带异种电荷的聚电解质，可形成多种功能超薄膜，其工艺简单，厚度可控，适用于具有复杂体型结构的材料，且制备条件温和，可实现多种生物分子的表面固定，实用性较强。

同行评价：实验选择具有良好生物活性的丝肽素和壳聚糖，通过层层静电自组装方法，尝试将这些物质有机地聚合在不锈钢材料上，探索一种制备带有生物活性膜的不锈钢支架材料的新方法，不仅具有一定的理论意义，更具有广阔的应用前景。

摘要
背景：金属支架本身的致血栓性使对其进行表面改性研究极具应用意义。
目的：利用层层静电自组装形成丝素肽壳聚糖聚合物膜。
设计、时间及地点：材料改性体外实验，于2006-07/2007-07在中国科学院上海生命科学研究院植物生理生态研究所实验室完成。
方法：通过层层静电自组装方法将丝素肽和壳聚糖交替涂层至医用316L不锈钢表面，对不锈钢表面进行改性。
主要观察指标：应用扫描电镜观察涂层前后316L不锈钢表面形貌变化，通过傅里叶红外光谱分析涂层前后波谱变化，测定材料表面接触角分析涂层结果，并进行体外降解实验观察涂层的稳定性。
结果：扫描电镜显示涂层后不锈钢表面均匀、致密；傅里叶变换红外光谱测定显示复合膜分子间存在强的离子键结合作用，也存在着强的氢键作用；接触角测定结果显示丝素肽和壳聚糖被交替涂层至不锈钢表面; 体外降解实验证实了涂层不锈钢的降解过程相对稳定。
结论：丝素肽与壳聚糖可通过静电自组装在不锈钢表面形成薄膜。
关键词：丝素肽；壳聚糖；静电自组装；316L不锈钢；生物材料；表面改性

李晓燕，蒋容静，刘建平，曹梅讯，李胜，郑莺，江柳月.丝素肽壳聚糖聚合物涂层316L不锈钢的制备[J].中国组织工程研究与临床康复，2008，12(14):2652-2655
[www.zglckf.com/zglckf/ejournal/upfiles/08-14/14k-2652(ps).pdf]

Preparation of silk peptide and chitosan polymer-coated 316L stainless steel

Abstract

BACKGROUND: The researches of metal stents' surface modification have a high applicable value for its thrombophilia.
OBJECTIVE: To prepare silk peptide and chitosan polymeric membrane by layer-by-layer electrostatic self-assembly multiplayer.
DESIGN ,TIME AND SETTING: The in vitro experiment of materials modification was performed at Laboratory of Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences between July 2006 and July 2007.
METHODS: Silk peptide and chitosan were coated on medical 316L stainless steel alternately by layer-by-layer electrostatic self-assembly multiplayer to modify stainless steel surface.
MAIN OUTCOME MEASURES: We observe the changes of 316L stainless steel surface by scanning electron microscope before and after it was coated, analyze spectra changes before and after 316L stainless steel was coated through Fourier Transform Infrared Spectrum Analysis Technique, analyze results of coatings by measuring surface contact angle and observe the stability of coatings through performing in vitro degradation experiment.
RESULTS: Scanning electron microscope displayed the surface of coated stainless steel was uniform and pyknic. Fourier transform infrared spectrometer measurement results displayed there were strong ion bonds and hydrogen bonds effects between compound membrane molecules. Contact angle measurement results displayed silk peptide and chitosan were coated on stainless steel surface alternately. Experiment of in vitro degradation verifies the process of stainless steel degradation is relatively stable.
CONCLUSION: Silk peptide and chitosan can fom coatings on the surface of 316L stainless steel by electrostatic self-assembly multiplayer.

Li XY, Jiang RJ,Liu JP,Cao MX, Li S, Zheng Y, Jiang LY.Preparation of silk peptide and chitosan polymer-coated 316L stainless steel.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(14):2652-2655
[www.zglckf.com/zglckf/ejournal/upfiles/08-14/14k-2652(ps).pdf]