Abstract

BACKGROUND:Most of the natural chitosan are shaped as powder and have small specific surface area. It is difficult to reclaim both vector and adsorbate by chitosan, thus limiting its application as an adsorbing vector.
OBJECTIVE: To analyze the optimal preparation technology of porous chitosan microspheres.
DESIGN, TIME AND SETTING: Repeated-measures experiment was carried out in the laboratory of Applied Chemistry, Shaanxi University of Technology from November 2006 to May 2007.
MATERIALS: Chitosan with a deacetylation degree of 95.62%.
METHODS: The reverse liquid phase suspension polymerization method was adopted in this study using cyclohexane as porogenic agent and glutaraldehyde as crosslinking agent. Cyclohexane and cutaraldehyde were added continuously, with the alterations of reaction temperature, stirring speed and extraction time.
MAIN OUTCOME MEASURES: The influence on the preparation of porous chitosan microspheres by the dosage of cyclohexane and glutaraldehyde, reaction temperature, stirring speed and extraction time was observed.
RESULTS: The optimal conditions of preparing porous chitosan microspheres, in which 90% particle diameters were 3-12 μm, included: reaction temperature 60-70 ℃, medium stirring speed 100-120 r/min, extraction time no less than 48 hours.
CONCLUSION: The preparation method used in this study has short time-consuming, the prepared chitosan microspheres are characteristic as micron-grade diameter, narrow distributing particle size and porosity.

College of Chemistry and Environmental Science, Shaanxi University of Technology, Hanzhong 723000, Shaanxi Province, China

Li Zhi-zhou★, Studying for master's degree, Associate professor, College of Chemistry and Environmental Science, Shaanxi University of Technology, Hanzhong 723000, Shaanxi Province, China
lizhizhou136@sina.
com

Supported by: a grant of Scientific Research of Shaanxi University of Technology, No. SLGQD0605*

Received: 2008-01-24
Accepted: 2008-03-01
(07-50-12-6927/Y)

Li ZZ.Optimal preparation conditions of porous chitosan microspheres. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(14): 2770-2772(China)
[www.zglckf.com/zglckf/ejournal/upfiles/ 08-14/14k-2770
(ps).pdf]

多孔壳聚糖微球的最佳制备条件*★

摘要
背景：天然状态的壳聚糖大部分为粉末状，比表面积小，作为吸附载体，使载体和吸附物都难以回收，从而限制其应用。
目的：分析制备多孔壳聚糖微球的最佳条件。
设计、时间及地点：重复测量设计，于2006-11/2007-05 在陕西理工学院应用化学实验室完成。
材料：壳聚糖脱乙酰度为95.62%。
方法：以环己烷作致孔剂，戊二醛作交联剂，采用反液相悬浮交联法，改变加入环己烷和戊二醛用量，调节反应温度，改变搅拌速度和提取时间。
主要观察指标：观察反应温度、提取时间、搅拌速度、环己烷用量和戊二醛用量对制备多孔壳聚糖微球的影响。
结果：多孔壳聚糖微球最佳制备条件为:添加10 mL环己烷，戊二醛2 mL，反应温度60~70 ℃，搅拌速度中速（100~120 r/min）时，提取时间不小于48 h，可得到90%粒径大小为3~12 μm的多孔壳聚糖微球。
结论：此制备方法具有耗时短，制备的壳聚糖微球粒径小、粒度分布窄、多孔性等特点。
关键词：多孔壳聚糖；微球；制备；生物材料

李志洲
陕西理工学院化学与环境科学学院，陕西省汉中市 723000
李志洲★，男，1969年生，陕西省汉中市人，汉族，陕西科技大学在读硕士，副教授，主要从事化工工艺开发与应用的研究。
陕西理工学院科研资助项目（SLGQD0605）*

中图分类号: R318.08 文献标识码: B 文章编号: 1673-8225(2008)14-02770-03
李志洲.多孔壳聚糖微球的最佳制备条件[J].中国组织工程研究与临床康复，2008，12(14):2770-2772
[www.zglckf.com/zglckf/ejournal/upfiles/08-14/14k-2770(ps).pdf]
(Edited by: Nie J/Yang Y/Wang L)