番茄红素对去势大鼠骨量和骨生物力学的影响*★

杨希重1，季翠杰2，陈晓亮1，李磊1

课题背景：此课题为民政部“十一五”课题项目，民人教科学[（2007）18-1-27]。由活性氧诱发的氧化应激与骨质疏松的发生有关，食物中的抗氧化剂能够降低体内的氧化应激反应，番茄红素作为自然界中的抗氧化剂能够减低人类某些与年龄老化有关的疾病的发生。

应用要点：国内外研究证实番茄红素在治疗癌症及心血管疾病方面有独特作用。本实验提示番茄红素能够增加骨量和骨强度。实验中番茄红素治疗组的骨密度和生物力学指标均低于雌激素治疗组，其原因可能如下：①番茄红素对骨质疏松的治疗作用比较温和，可能需长时间用药。②样本例数不多，统计学检验效能不足。

同行评价：①课题主攻方向较陈旧，但施加的因素较先进。②设计合理，有一定创新性和科学性。③骨质疏松症是一种全身性疾病，它的治疗不仅仅是抗氧化的问题。
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摘要
目的：番茄红素作为自然界中的抗氧化剂，能够减低人类某些与年龄老化有关的疾病的发生。实验拟进一步验证番茄红素对实验性骨质疏松大鼠骨密度和骨生物力学的影响。
方法：实验于2006-07/2007-11在青岛大学医学院附属医院动物实验基地完成。番茄红素油树脂由新疆智星科技投资开发有限公司提供，使用时玉米油稀释至所需浓度。选择6个月龄SPF级雌性wistar大鼠50只，按体质量随机分成5组，即假手术+玉米油组 (2 mL/d)、卵巢切除+玉米油组 (2 mL/d)、卵巢切除+苯甲酸雌二醇组 (0.2 mg/kg,1次/周)、番茄红素低剂量组 (10 mg/kg，1次/ d)、番茄红素高剂量组 (20 mg/kg，1次/ d)。每组10只，除假手术组外其余各组均切除双侧卵巢，建立骨质疏松动物模型，按组给予灌服给药，治疗12周。
结果：术后2周内假手术组死亡1只，低剂量组死亡2只。47只大鼠进入统计学分析。①卵巢切除组大鼠腰椎及股骨的骨密度和最大应力均较假手术组降低，差异有显著性(P < 0.05)。②摄取番茄红素和雌激素的大鼠，股骨及腰椎骨密度和最大应力比卵巢切除组明显升高，差异有显著性(P < 0.05)。③番茄红素增加骨密度和提高最大应力作用不如雌激素明显；高剂量组作用强于低计量组。
结论：番茄红素可提高骨质疏松大鼠的骨密度和骨生物力学特性，但其作用低于雌激素，是否与用药时间和样本例数不多有关尚需观察。
关键词：番茄红素；骨质蔬松；骨密度；生物力学；组织构建

杨希重，季翠杰，陈晓亮，李磊. 番茄红素对去势大鼠骨量和骨生物力学的影响[J].中国组织工程研究与临床康复，2008，12(15):2811-2814 [www.zglckf.com/zglckf/ejournal/upfiles/08-15/15k-2811(ps).pdf]

1青岛大学医学院附属医院脊柱外科，山东省青岛市 266003；2蓬莱市第二人民医院内科，山东省蓬莱市 265612

杨希重★，男，1972年生，山东蓬莱市人，汉族，青岛大学在读硕士，主治医师，主要从事脊柱外科的研究。
yanyubaihe@126.com

通讯作者：陈晓亮，硕士，教授，硕士生导师，青岛大学医学院附属医院脊柱外科，山东省青岛市
266003

民政部十一五课题项目，民人教科学[(2007)18-1-27]*

中图分类号:R681.4
文献标识码:A
文章编号:1673-8225
(2008)15-02811-04

收稿日期：2007-12-28 修回日期：2008-02-21
(07-50-10-5711/W·A)

Abstract

AIM：Lycopene as an antioxidant can decrease the risk of age-related chronic diseases, such as cancer. In this study, we investigated the impact of lycopene on bone mineral density and bone biomechanics in experimental osteoporotic rats.
METHODS: The experiment was performed at the Animal Experiment Center of the Affiliated Hospital of Qingdao University Medical College from July 2006 to November 2007. The lycopene was provided by the Xinjiang Zhixing Technology Invest Development Company, and diluted to certain concentration by corn oil. Fifty six-month-old SPF female Wistar rats were selected and divided into 5 groups (n=10) according to body mass: Sham operation and corn oil group (2 mL/d), ovariectomy (OVX) and corn oil group (2 mL/d), OVX and estradiol benzoate (EB) group (0.2 mg/kg, once a week), low and high lycopene groups (10 mg/kg, 20 mg/kg, once a day). Except the sham operation group, all rats underwent OVX to establish models of osteoporosis. Each group was administrated corresponding medicine for 12 weeks.
RESULTS: Within 2 weeks postoperatively, 1 of sham operation group died and 2 of low lycopene group died. Finally, 47 rats were included in final analysis. ①The lumbar and femoral bone mineral density and maximum stress of ovariectomized rats were significantly lower than those of sham operation group (P < 0.05). ②The lumbar and femoral bone mineral density and maximum stress of rats treated by lycopene and EB were significantly greater than those in ovariectomized rats (P < 0.05). ③Lycopene could increase bone mineral density and maximum stress, but its effect was inferior to EB. In addition, high dose group had stronger effect compared with low dose group.
CONCLUSION: Lycopene can improve the bone mineral density and biomechanics of ovariectomized rats, but the effect is lower than EB, which may be correlated with administration time and sample quantity.

Yang XZ, Ji CJ, Chen XL, Li L. Effect of lycopene on bone mass and biomechanics in ovariectomized rats.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(15):2811-2814(China)
[www.zglckf.com/zglckf/ejournal/upfiles/08-15/15k-2811(ps).pdf]

1Department of Spinal Surgery, Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China; 2Department of Internal Medicine, Penglai Second People's Hospital, Penglai 265612, Shandong Province, China

Yang Xi-zhong★, Studying for master's degree, Attending physician, Department of Spinal Surgery, Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China
yanyubaihe@126.com

Correspondence to: Chen Xiao-liang, Master, Professor, Tutor of master, Department of Spinal Surgery, Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China

Supported by: the Program of Ministry of Civil Affairs during the Eleventh-Five-Year plan period, No. (2007) 18-1-27*

Received: 2007-12-28
Accepted: 2008-02-21