精索静脉曲张管壁肌动蛋白和胶原的定性与定量分析
刘学银，苟 欣
应用要点：本文从超微结构、免疫组织化学等方面对曲张的精索静脉进行了观察；并从另一角度采用图像分析方法进行平均吸光度及平均面密度值分析，丰富了血管重塑的病程、细胞、分子等方面的资料。
同行评价：文章通过对精索静脉曲张管壁的肌动蛋白及胶原纤维的定性和定量研究，以探讨血管重塑在精索静脉曲张发生机制中的意义，目的明确。具有一定的创新性和科学意义。
相关链接：目前，众多国内外学者对于血管重塑有大量的研究报道。由河北医科大学教授韩梅等人完成的“血管重塑性疾病的细胞与分子机制研究”，从细胞和分子生物学角度对血管重塑性疾病的发生机制和发展规律提出了新的观点和试验数据，为该类疾病的治疗和新药研究机制提供了新的切入点。本领域有待解决的问题:①进一步研究精索静脉曲张血管重塑的发病机制。②找出治疗和研究新药治疗精索静脉曲张的切入点。
摘要
背景：目前关于心脏、大血管疾病的血管重塑及其病理学、分子生物学、免疫组织化学以及药物对血管重塑的影响有大量的研究报道，而对于精索静脉曲张的有关报道较少。
目的：观察精索静脉曲张过程中管壁肌动蛋白和胶原的定性与定量的变化。
设计、时间及地点：同期非随机对照观察，于2006-03/2007-06在重庆医科大学附属第一医院泌尿外科完成 。
对象：实验组精索静脉取自2006-03/2007-06在重庆医科大学附属第一医院泌尿外科接受手术治疗的精索静脉曲张患者30 例；年龄(26.29±9.12)岁；病程(2.76±1.83)年。对照组选取同期该科肾移植供体正常精索静脉，共24 例，年龄(39. 56 ±8.32)岁。患者及供体亲属对治疗完全知情同意。
方法：采用V-G染色、免疫组织化学、扫描电镜定性和定量观察30 例曲张精索静脉和24例正常精索静脉壁中肌动蛋白及胶原纤维变化，并进行图像分析。
主要观察指标：两组精索静脉平滑肌细胞、胶原纤维、Ⅰ型胶原、Ⅲ型胶原和α-肌动蛋白的变化及吸光度和面密度的变化。
结果：①曲张精索静脉内膜、中膜改变以增厚为主，平滑肌细胞从收缩表型向合成表型转化。②曲张静脉α-肌动蛋白的吸光度和面密度均比正常静脉增多（P < 0. 01）；曲张静脉Ⅰ型胶原及Ⅲ型胶原的吸光度和面密度均比正常静脉增多（P < 0.01）。
结论：精索静脉曲张管壁的平滑肌细胞异常增生，从收缩表型向合成表型转化，细胞外基质中胶原异常改变，可能是精索静脉曲张发生和发展的机制之一。
关键词：静脉曲张；肌，平滑；血管/细胞学；胶原；肌动蛋白类；血管构建
刘学银，苟欣. 精索静脉曲张管壁肌动蛋白和胶原的定性与定量分析[J].中国组织工程研究与临床康复，2008，12(15):2859-2862 [www.zglckf.com/zglckf/ejournal/upfiles/08-15/15k-2859(ps).pdf]
重庆医科大学附属第一医院泌尿外科，重庆市
400016
刘学银，男，1969年生，重庆市巫溪县人，汉族，1993年重庆医科大学毕业，副主任医师，主要从事男性生殖的研究。
wxlxy2008@163.com
通讯作者：苟 欣，硕士，教授，主任医师，硕士生导师，重庆医科大学附属第一医院泌尿外科，重庆市 400016
cymnk@163.com
中图分类号:R329.412
文献标识码:B
文章编号:1673-8225
(2008)15-02859-04
收稿日期：2008-01-18 修回日期：2008-03-12 (08-50-1-508/W·Y)
Abstract
BACKGROUND: At present, there are considerable investigations and reports concerning the vascular remodeling cum pathology, molecular biology, immunohistochemistry, drug effect to the disease of the heart and great vessels. But varicocele is seldom reported.
OBJECTIVE: To observe the change of the qualitation and quantitation of actin and collagen in varicocele vessel wall.
DESIGN, TIME AND SETTING: A synchronized non-randomized control observation was carried out in the Department of Urinary Surgery, the First Affiliated Hospital of Chongqing University of Medical Sciences from March 2006 to June 2007.
PARTICIPANTS: Thirty varicocele patients treated in Department of Urinary Surgery, the First Affiliated Hospital of Chongqing University of Medical Sciences from March 2006 to June 2007, were recruited for experimental group in this study. Their age was (26.29±9.12) years, and the course of disease was (2.76±1.83) years. Meanwhile, 24 normal spermatic veins from the donator of kidney transplantation, aged (39.56±8.32) years, were served as control group. All the patients and the donators' relatives signed the informed consent to the treatment.
METHODS: Changes of collagen fibers and actin in 24 normal spermatic veins and 30 varicocele veins were studied quantitatively and qualitatively by the methods of V-G staining, immunohistochemistry, and scanning electron microscopy. The image analysis was conducted.
MAIN OUTCOME MEASURES: Changes of smooth muscle cells (SMC), collagen fibers, type Ⅰ collagen, type Ⅲ collagen and α-smooth muscle actin, as well as optical density and area density.
RESULTS: ①Tunica intima hyperplasia and medial thickening were the main histological appearances of varicocele veins, SMC transformed from the contractile phenotype to the synthetic phenotype.②The optical density and area density of α-actin, type Ⅰ collagen and type Ⅲ collagen were increased significantly compared with the normal veins (P < 0.01).
CONCLUSION: The development of varicocele may be associated with paraplastic SMC transforming from the contractile phenotype to the synthetic phenotype, and the abnormal change of collagen in the extracellular matrix.
Liu XY, Gou X. Qualitative and quantitative analysis of actin and collagen in varicocele vessel wall.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(15):2859-2862(China)
[www.zglckf.com/zglckf/ejournal/upfiles/08-15/15k-2859(ps).pdf]
Department of Urinary Surgery, the First Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400016, China
Liu Xue-yin, Associate chief physician, Department of Urinary Surgery, the First Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400016, China
wxlxy2008@163.com
Correspondence to: Gou Xin, Master, Professor, Chief physician, Tutor of master, Department of Urinary Surgery, the First Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400016, China
cymmk@163.com
Received: 2008-01-18
Accepted: 2008-03-12
Advertisement |
 |