Abstract

BACKGROUND:Researches on establishing restenosis models in rats emphasize the importance of 2.0 Forgarty balloon catheter import, but little has been mentioned about domestic-made balloon catheter.
OBJECTIVE: To explore the characteristics of vessel restenosis model by denuding arterial endothelium with domestic-made 2.0 Forgarty balloon catheter in rats.
DESIGN: A randomized controlled animal experiment.
SETTING: Laboratory of Pathophysiology, Hunan University of Traditional Chinese Medicine.
MATERIALS: This study was performed at the Laboratory of Pathophysiology, Hunan University of Traditional Chinese Medicine between September 2006 and January 2007. Healthy male SD rats, weighing 300-350 g, were selected. The protocol was performed in accordance with ethical guidelines for the use and care of animals. Domestic-made 2.0 Forgarty balloon catheters were made of balloons and catheters. Proliferating cell nuclear antigen (PCNA), collagen I, and SMα-actin immunohistochemical staining kits were provided by Wuhan Boster Bioengineering Co., Ltd, China.
METHODS: The rats were randomly divided into 4 groups: sham-operated group (n = 5), 7th day model group (n = 4), 14th day model group (n = 5), and 21st day model group (n = 4). The vessel restenosis models were established in the latter 3 groups by denuding arterial endothelium with domestic-made balloon catheter in rats. In the sham-operated group, rats were without insertion of balloon catheter. Injured thoracic aortas were taken out for HE staining to observe histological changes of vascular intima. At the meantime, expressions of SMα-actin, PCNA and collagen I were determined by immunohistochemical method in each group.
MAIN OUTCOME MEASURES: Histological changes of intima at the thoracic aorta as well as expressions of SMα-actin, PCNA and collagen I.
RESULTS: Eighteen rats were included in the final analysis. The aortal vascular walls remained integrity without narrowness of the lumen areas and proliferation of the endothelium, vascular smooth muscle cells (VSMCs) ranged regularly in the mesolamella in the sham-operated group. The endothelium had little proliferation in the 7th day model group. The intima thickened and the area of lumen became narrow in the 14th day model group. The intimal hyperplasia was diffusive and progressive which mainly included VSMCs, the area of lumen narrowed markedly and cells ranged disorderly in mesolamella in the 21st day model group. In the 14th day model group, PCNA level was higher compared to the remaining 3 groups (P < 0.01). SMα-actin level were significantly higher in the 14th and 21st day model groups than in the sham-operated group and 7th day model group (both P < 0.01). There were no significant differences in collagen I among the groups (P > 0.05).
CONCLUSION: Vascular intima hyperplasia appeared markedly 14-21 days after domestic-made balloon catheter-induced rat aorta injury. The results suggested that intima hyperplasia is mainly induced by proliferation of VSMCs.

1Hunan University of Chinese Medi-cine, Changsha 410007, Hunan Province, China; 2Laboratory for Pathophysiology, Hunan University of Chinese Medicine, Changsha 410007, Hunan Province, China

Wu Lu★, Studying for master’s degree, Hunan University of Chinese Medicine, Changsha 410007, Hunan Province, China wl09131202@163.
com

Correspondence to: Deng Chang-qing, Doctor, Professor, Laboratory for Pathophysiology, Hunan University of Chinese Medicine, Changsha 410007, Hunan Province, China dchangq@sohu.com

Supported by: the National Natural Science Foundation of China, No. 30572301*

Received:2007-10-26
Accepted:2007-12-07
(07-50-10-5851/YWY)

Wu L, Zhang W, Deng CQ. Charac-teristics and feasibil-ity of vessel restenosis models produced by denud-ing arterial endothe-lium with domes-tic-made balloon catheter in rats. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(17):3372-
3375(China)

[www.zglckf.com/
zglckf/ejournal/
upfiles/08-17/
17k-3372(ps).pdf]

国产球囊导管制作大鼠主动脉损伤血管
再狭窄模型的特性及可行性*★

摘要
背景：研究应用国产球囊导管可否替代2.0F进口球囊导管制作大鼠主动脉损伤血管再狭窄模型。
目的：验证国产2.0F球囊导管致大鼠主动脉损伤血管再狭窄模型的特点及可行性。
设计：随机对照动物实验。
单位：湖南中医药大学病理生理学实验室。
材料：实验于2006-09/2007-01在湖南中医药大学病理生理学实验室完成。选用健康雄性SD大鼠，体质量300~350 g。实验过程中对动物的处置符合动物伦理学标准。国产2.0F球囊导管由球囊和导管组装而成。增殖细胞核抗原、Ⅰ型胶原、SMα-actin免疫组化染色试剂盒由武汉博士德生物工程有限公司提供。
方法：①随机数字表法法将大鼠分为假手术组(n = 5)、模型7 d组(n = 4)、模型14 d组(n = 5)和模型21 d组(n = 4)，后3组大鼠经麻醉后采用国产2.0F球囊导管建立大鼠主动脉内皮剥脱模型，假手术组只进行颈总动脉近心端切口，但不插入球囊损伤。②模型7，14，21 d组大鼠于术后7，14，21 d取损伤部位胸主动脉段进行切片及HE染色，观察大鼠血管内膜组织学变化，同时采用免疫组织化学法检测血管平滑肌细胞表型标志物SM-肌动蛋白、增殖细胞核抗原及Ⅰ型胶原表达情况。假手术组于干预后直接取材观察，检测项目同各模型组。
主要观察指标：大鼠胸主动脉段内膜组织学变化及SM-肌动蛋白、增殖细胞核抗原和Ⅰ型胶原表达。
结果：纳入大鼠18只均进入结果分析。①假手术组各层结构完整，管腔面积无缩小，内膜光滑无增生，中膜血管平滑肌细胞排列整齐。模型7 d组增生不明显，模型14 d组内膜有增厚，管腔面积有缩小，模型21 d组内膜呈进行性弥漫性增厚，管腔面积明显缩小，增厚的内膜中以平滑肌细胞为主，中膜细胞排列紊乱。②模型14 d组增殖细胞核抗原水平高于其他3组(P < 0.01)， 样型14 d组21 d组SM-肌动蛋白含量高于；模型21 d组SM-肌动蛋白含量低于假手术组及模型7 d组，差异均有显著性意义（P < 0.01）；各组Ⅰ型胶原表达水平差异均无显著性意义(P > 0.05)。
结论：国产2.0F球囊导管致大鼠主动脉损伤后14~21 d可出现明显血管内膜增生，该增生主要是由血管平滑肌细胞增殖所致。
关键词： 球囊导管；血管平滑肌细胞； SM-肌动蛋白；增殖细胞核抗原；Ⅰ型胶原

吴 露1，张 伟1，邓常青2
1湖南中医药大学2005级研究生班，湖南省长沙市 410007; 2湖南中医药大学病理生理学实验室，湖南省长沙市 410007
吴 露★，女，1981年生，陕西省安康市人，汉族，湖南中医药大学在读硕士，助教，主要从事心脑血管疾病防治机制的研究。
通讯作者：邓常青，博士后，教授，湖南中医药大学病理生理学实验室，湖南省长沙市 410007
国家自然科学基金资助项目(30572301)*

中图分类号: R318 文献标识码: B 文章编号: 1673-8225(2008)17-03372-04
吴露，张伟，邓常青.国产球囊导管制作大鼠主动脉损伤血管再狭窄模型的特性及可行性[J].中国组织工程研究与临床康复，2008，12(17):3372-3375
[www.zglckf.com/zglckf/ejournal/upfiles/08-17/17k-3372(ps).pdf]
（Edited by Ding C/Cui X/Song LP/Wang L）