Effect of water decoction of the Gastrodia elata Bl on vasodilation of rabbit aorta in vitro
Zhang Tuan-xiao1, Niu Cai-qin2, Jing Hua-e1, Liu Hong1
Abstract
BACKGROUND:Recent research has shown that Gastrodia elata Bl (GEB) has a cardiovascular protective effect and relaxes blood vessels with important therapeutic implication to treat coronary heart disease and hypertension. However, the mechanism is still unclear.
OBJESTIVE: To study the effect of the water decoction of GEB on noradrenaline (NA) or KCl precontracted aortic rings and the possible mechanisms.
DESIGN: A grouping observational experiment of animal tissue in vitro.
SETTING: Department of Physiology, North Sichuan Medical College.
MATERIALS: Twelve health New Zealand White rabbits (2.5-3.0 kg, 7-8 months, SPF, either gender) were provided by the Experimental Animal Center, North Sichuan Medical College. The water decoction of GEB was prepared by Huirentang Drugstore and diluted into 10%, 20%, 40% and 80% solution. The following drugs were used: Nω-nitro-L-arginine (L-NNA, Sigma, USA); Methylene blue (MB, Merck, Germany); Acetylcholine (ACh) and Propranolol (Prop) (The Second Beijing Pharmaceutical Company, China); Indomethacin (Indo, Jingsu Taicang Pharmaceutical Company, China).
METHODS: The experiment was performed in the Institute of Materia Medica, North Sichuan Medical College between January 2006 and January 2007. Rabbit smooth muscles of aortic rings were isolated and precontracted with NA. The thoracic aortic rings were treated with GEB with cumulative concentrations of 1.0, 2.0, 4.0, 8.0 and 16 g/L respectively. The aortic rings were treated with one of the following signaling inhibitors for 15 minutes, including 1×10-4 mol/L L-NNA, 1×10-5 mol/L MB, 1×10-5 mol/L Indo and 1×10-5 mol/L Prop. The changes of tension in aortic rings were recorded using a force transducer and processed by BL-410 Experimental System of Biological Function. The aortic rings were incubated with 8 g/L GEB followed by the NA and KCl dose response experiments.
MAIN OUTCOME MEASURES: Blood vessel tension ex vivo.
RESULTS: GEB did not change the resting tension of rabbit aortic rings, but GEB treatment resulted in an obvious relaxation in NA-precontracted aortic rings (r =0.85, t=18.45, P < 0.01) and the relaxant effect was dose-dependent. The relaxant effect of GEB was significantly reduced by removal of endothelium and by treatment with 1×10-4 mol/L nitric oxide synthase inhibitor L-NNA (1×10-4 mol/L), or 1×10-5 mol/L guanylyl cyclase inhibitor methylene blue (1× 10-5 mol/L MB), but not by treatment with prostaglandin synthase inhibitor or blockage of the adrenergic β-receptor (1×10-5 mol/L Prop). In addition, GEB (8 g/L) decreased the dose response curves of aortic rings to NA or KCl in the absence of endothelial cells, and changed the PD2 values for NA from (6.90±0.93) mol/L in control group to [(5.61±0.70) mol/L, t =2.41, P < 0.05] and for KCl from (1.53±0.55) mmol/L in control group to (1.10±0.25) mmol/L, t =3.82, P < 0.05] respectively.
CONCLUSION: GEB can relax isolated rabbit aorta not only in an endothelium-dependent, nitric oxide involved manner, but also is related to blockage of receptor-operated and potential-dependent calcium channels.
1Department of Physiology, 2Clinical Department of Western Medicine and Traditional Chinese Medicine, North Sichuan Medical College, Nanchong 637007, Sichuan Province, China
Zhang Tuan-xiao, Associate professor, Department of Physiology, North Sichuan Medical College, Nanchong 637007, Sichuan Province, China
Received: 2007-10-22 Accepted: 2007-12-04 (07-50-9-5260/H)���������
Zhang TX, Niu CQ, Jing HE, Liu H. Effect of water decoction of the Gastrodia elata Bl on vasodilation of rabbit aorta in vitro. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(20):3988-
3991 (China)
[www.zglckf.com/
zglckf/ejournal/
upfiles/08-20/
20k-3988(ps).pdf]
家兔离体主动脉血管对天麻水煎剂干预的反应
摘要
背景：现代医学研究发现天麻（Gastrodia elata Bl）具有明显地心肌保护和血管扩张作用，可用于冠心病、高血压的治疗，但其作用机制仍需明确。
目的：观察天麻水煎剂对去甲肾上腺素、KCl预收缩血管反应的舒张作用，并分析其作用途径。
设计：分组设计、离体动物组织对照实验。
单位：川北医学院生理学教研室。
材料：选用健康家兔12只，兔龄七、八个月，雌雄不拘，由川北医学院实验动物中心提供。实验所用药品天麻水煎剂由慧仁堂药店鉴定提取，将其配制成10%，20%，40%和80%浓度备用。β肾上腺素能受体阻断剂普萘洛尔为北京第二制药厂产品；一氧化氮合酶抑制剂左旋硝基精氨酸为Sigma公司产品；甲烯蓝、环氧酶抑制剂吲哚美辛为江苏太仓制药厂产品。
方法：实验于2006-01/2007-01在川北医学院药物研究所完成。采用家兔离体主动脉平滑肌标本，以去甲肾上腺素(1×10-6 mol/L)预收缩主动脉后给予1.0，2.0，4.0，8.0和16.0 g/L天麻水煎剂观察其张力变化，并观察去除血管内皮、给予1×10-4 mol/L左旋硝基精氨酸、1×10-5 mol/L甲烯蓝、1×10-5 mol/L吲哚美辛和1×10-5 mol/L普萘洛尔对血管张力的影响；血管张力经张力换能器输入BL-410智能型生物信号处理系统进行处理。另外观察8 g/L天麻水煎剂对无内皮细胞血管环NA和KCl的量效收缩反应。
主要观察指标：离体血管张力。
结果：天麻对血管环静息张力无明显影响，但不同剂量的天麻可使1×10-6 mol/L 去甲肾上腺素预收缩血管产生明显舒张（r=0.85, t=18.45, P < 0.01）。去除血管内皮、1× 10-4 mol/L 左旋硝基精氨酸或1×10-5 mol/L 甲烯蓝可减弱天麻的舒张作用，但吲哚美辛和普萘洛尔对其无影响。另外，8 g/L天麻水煎剂对无内皮细胞血管环去甲肾上腺素和KCl的量效收缩反应明显降低，且去甲肾上腺素和KCl的PD2（-log游离药物半数有效浓度）分别由温育前（6.90±0.93）mol/L和（1.53± 0.55） mmol/L降低为（5.61±0.70）mol/L和(1.10±0.25）mmol/L, (t=2.41，3.82, P < 0.05)。
结论：天麻水煎剂对主动脉的舒张是内皮依赖性的，并与内皮一氧化氮的释放有关；也可通过拮抗ROC和PDC通道，抑制Ca2+的内流和释放等机制有关。
关键词：主动脉平滑肌；内皮细胞；血管舒张；天麻
张团笑1,牛彩琴2,敬华娥1 ,刘 红1 (川北医学院，1生理学教研室，2中西医临床系，四川省南充市 637007)
张团笑，男，1968年生，甘肃省静宁县人，2005年兰州大学毕业，副教授，主要从事心血管药理与生理的研究。
中图分类号: R329.41 文献标识码: A 文章编号: 1673-8225(2008)20-03988-04
张团笑, 牛彩琴, 敬华娥, 刘红. 家兔离体主动脉血管对天麻水煎剂干预的反应[J].中国组织工程研究与临床康复,2008,12(20): 3988-3991
[www.zglckf.com/zglckf/ejournal/upfiles/08- 20/20k-3988(ps).pdf]
(Edited by Yu XP/Ji H/Wang L)
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