课题背景：同种或异种肝细胞移植过程中发生免疫排斥反应会影响细胞移植的效果，微胶囊作为一种重要的免疫隔离工具可显著降低免疫排斥反应。但微胶囊内的高渗透压胁迫环境对动物细胞生长有一定的影响。本文旨在考察抗渗透压胁迫物质牛磺酸是否可改善微囊化肝细胞的生长，以期为微囊化生物人工肝的构建提供实验基础。

应用要点：实验观察到：①牛磺酸对非微囊化肝细胞的生长无显著影响。②1.2 mmol/L牛磺酸可以显著促进微囊化肝细胞的生长，并促进细胞的聚集。实验结果为改善微囊化动物细胞生长提供了性的途径。

同行评价：实验针对微囊化细胞的培养方法进行探索，具有一定的理论意义和潜在应用价值，也有一定的创新性。

摘要
目的: 微胶囊中存在一定程度的高渗透压胁迫，可造成细胞生长代谢减慢，为改善微囊化肝细胞的生长，尝试应用抗渗透压胁迫物质牛磺酸，观察其对微囊化肝细胞生长的影响。
方法: 实验于2006-07/08在中国科学院大连化学物理研究所生物医学材料工程实验室完成。将HepG2细胞悬液(非微囊化细胞)和微囊化HepG2分别接种于牛磺酸浓度为0，0.6，0.8和1.2 mmol/L的MEM培养液中，在37 ℃体积分数为0.05的CO2中培养。MTT法测定非微囊化和微囊化HepG2细胞的生长活性，相差显微镜观察囊内细胞生长状态。
结果：①非微囊化HepG2细胞：0.6，0.8和1.2 mmol/L的牛磺酸对其生长无影响，吸光度值比较差异无显著意义（P > 0.05）。②微囊化HepG2细胞：0.6，0.8 mmol/L牛磺酸对微囊化肝细胞的生长亦无影响（P > 0.05）；但1.2 mmol/L牛磺酸可以显著改善微囊化肝细胞的生长，其吸光度值高于0 mmol/L牛磺酸组（P < 0.05），并促进细胞的聚集。
结论：1.2 mmol/L牛磺酸可以改善微囊化肝细胞的生长并抵制微囊内的高渗透压胁迫对细胞的生长抑制作用。
关键词：微囊化；牛磺酸；渗透压胁迫；肝细胞；生长；生物材料

孙志杰，郭昕，宁小娟，李双月，吕国军，于炜婷，王为，马小军.牛磺酸对微囊化肝细胞生长的影响[J].中国组织工程研究与临床康复，2008，12(6):1031-1034 [www.zglckf.com/zglckf/ejournal/upfiles/08-6/6k-1031(ps).pdf]

Influence of taurine on the growth of microencapsulated hepatocytes

Abstract

AIM：Hyper-osmotic stress may occur in the microcapsules, and reduce the metabolism of cells. This study was designed to investigate the influence of taurine, as an osmolarity substance, on the growth of microencapsulated hepatocytes.
METHODS: The experiment was done in the Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics of the Chinese Academy of Sciences between July and August in 2006. Suspended HepG2 cells (control group) and microencapsulated HepG2 cells were inoculated into MEM mediums which contained 0, 0.6, 0.8 and 1.2 mmol/L taurine and cultured at 37 ℃ in an atmosphere with 0.05 volume fraction of CO2. Growth activities of suspended HepG2 cells and microencapsulated HepG2 cells were determined by MTT methods, and the growth of microencapsulated HepG2 cells was observed under phase-contrast microscopy.
RESULTS: ①Taurine at 0.6, 0.8 and 1.2 mmol/L had insignificant effects on the growth of non-encapsulated HepG2 cells. There was no significant difference in the absorptance value (P > 0.05).②Taurine at 0.6 and 0.8 mmol/L did not alter the growth of microencapsulated cells (P > 0.05), but taurine at 1.2 mmol/L increased the growth activities and prompted cell aggregation of microencapsulated HepG2 cells. The absorbtance value was also higher compared to that in 0 mmol/L taurine treatment (P < 0.05).
CONCLUSION: Taurine at 1.2 mmol/L can significantly facilitate the growth of microencapsulated HepG2 cells and can prevent the inhibition effects of hyper-osmotic stress on cellular growth.

Sun ZJ, Guo X, Ning XJ, Li SY, Lü GJ, Yu WT, Wang W, Ma XJ.Influence of taurine on the growth of microencapsulated hepatocytes.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(6):1031-1034(China)
[www.zglckf.com/zglckf/ejournal/upfiles/08-6/6k-1031(ps).pdf]