课题背景：课题由中国意大利政府合作项目（2005GH22）支持。魔芋葡甘聚糖是一类新兴的生物高分子材料。已在可控释载体、皮肤组织工程材料等领域获得诸多应用，但应用于骨组织工程材料的研究仍少有报道。

应用要点：①魔芋葡甘聚糖是支链高分子，比胶原、壳聚糖、丝蛋白和纤维素等直链高分子可以获得更佳的力学性能和成型性能。②可以通过控制魔芋葡甘聚糖的脱乙酰度控制其和羟基磷灰石的复合方式以及复合程度。③魔芋葡甘聚糖不会引起机体免疫反应。④魔芋葡甘聚糖成本低廉。

相关链接：魔芋葡甘聚糖是一种从魔芋块茎中提取的由D-甘露糖和D-葡萄糖通过β－1,4吡喃糖苷键连接而成中性多糖，可形成具有三维网络结构的热稳定性凝胶。魔芋葡甘聚糖还是一种pH敏感性多糖，可以通过调节系统的pH值来控制其凝胶促发时间和凝胶程度，这种特性对控制其机械性能、生物降解性能及其与其他材料的复合性能非常有利。利用魔芋葡甘聚糖可获得孔结构丰富且孔径可调的三维网状结构，具有良好的持水能力、生物相容性和降解性。

摘要
目的: 制备羟基磷灰石/魔芋葡甘聚糖复合材料，分析两者间的结合机制以及羟基磷灰石和魔芋葡甘聚糖复合性能最佳时的工艺条件。
方法：实验于2007-03/06在昆明理工大学组织工程支架与多孔催化载体实验室完成。通过共沉淀法制备羟基磷灰石(hydroxyapatite，HAP)/魔芋葡甘聚糖(konjac glucomannan，KGM)复合材料。对不同pH样品进行X射线衍射仪物相分析。KBr压片法制样，傅里叶转换红外光谱仪测定样品的傅里叶转换红外光谱。样品表面喷金后，用扫描电镜观察样品的形貌和微观结构及进行电子能谱分析。
结果：复合材料中的无机相为部分碳酸根取代的呈弱结晶状态的HAP, KGM分子链上乙酰基中的羰基是HAP的成核位点。pH值是影响HAP和KGM间交互作用的关键因素，HAP和KGM的原料比对复合性能有明显影响。当pH=9.0和10.7、原料比为w（HAP/KGM）=30/70时，HAP和KGM复合良好。
结论：实验制备的HAP/KGM复合材料是一种潜在的骨组织工程支架材料，pH值和HAP/KGM原料比是影响HAP和KGM 复合性能的重要影响因素。
关键词：复合材料；羟基磷灰石；魔芋葡甘聚糖；pH值；材料组成；生物材料

张峰，陈庆华，潘兴华，庞荣清，景森，王大为.羟基磷灰石/魔芋葡甘聚糖复合材料的制备和表征[J].中国组织工程研究与临床康复，2008，12(6):1072-1076 [www.zglckf.com/zglckf/ejournal/upfiles/08-6/6k-1072(ps).pdf]

Preparation and characterization of hydroxyapatite/konjac glucomannan composites

Abstract

AIM：To prepare the hydroxyapatite (HAP)/konjac glucomannan (KGM) composite materials, analyze the integrating mechanism of HAP and KGM, and explore the optimal technique condition for the best performance.
METHODS: The experiment was carried out in the Laboratory of Tissue Engineering Scaffolds and Porous Catalytic Carriers from March to June in 2007. HAP/KGM composites were synthesized by the coprecipitation method. X-ray diffraction analyzer was used for difficult pH values of samples. Sampling was conducted by using KBr pellet method, and detected by using fourier transform infrared spectroscopy. After the samples were metallized by spraying on surface, scanning electron microscope was employed to characterize the composites, including morphology, microstructure and electron spectrum.
RESULTS: The inorganic phase in composites was partially carbonate-substituted HAP with low crystallinity, and the carbonyl groups of acetyl groups on the surface of KGM molecules were the nucleation sites of HAP. The pH value was a key influencing factor for the phase interaction between HAP and KGM, and the raw material ratio of HAP/KGM was also important to determine the composite performance of HAP/KGM composites. The two phases of HAP and KGM combined well while pH values were 9.0 and 10.7, the raw material ratio of HAP/KGM was 30/70.
CONCLUSION: This prepared HAP/KGM composites will be a potential biomaterial as bone tissue engineering scaffold, and the composite performance can be influenced by the pH value and the raw material ratio of HAP/KGM.

Zhang F, Chen QH, Pan XH, Pang RQ, Jing S, Wang DW.Preparation and characterization of hydroxyapatite/konjac glucomannan composites.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(6):1072-1076(China)
[www.zglckf.com/zglckf/ejournal/upfiles/08-6/6k-1072(ps).pdf]